产品基本信息

• B3808
• Flag-Tag (12M11) Mouse Monoclonal Antibody
• 抗原抗体
• Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
• Synthetic Peptide of Flag-Tag
• This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library i
• WB 1:1000-2000 IF 1:200 IHC 1:50-300
• -15°C to -25°C/1 year(Do not lower than -25°C)
• Mouse VH IgG1 and VL Kappa was expressed from 293F cells
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• >95% SDS-PAGE

图片

	RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Zhihua Liu WB,IP Human 1:5000 HEK 293 T cell,KYSE30 cell,KYSE450 cell
	Western Blot analysis of 293F cells transfected or non-tansfected DDDK-tag expression vector by primary antibody at 1:10000 dilution. Lane 1 3 5: 293F cell lysis. Lane 2 4 6: tansfected DDDK-tag expression vector. Secondary antibody was diluted at 1:10000
	Western Blot analysis of various cells using primary antibody at 1:10000 dilution. Secondary antibody was diluted at 1:10000
	1) Input: 293T transfected N-flag protein Lysate 2) IP product: IP dilute 1: 200, 293T transfected N-flag protein Lysate Western blot analysis: primary antibody : 1:10000 Secondary antibody: Goat anti-Mouse IgG, 1: 5000
	Immunofluorescence analysis of 293T transfected FLAG and GFP tag protein. 1, FLAG primary Antibody was diluted at 1:1000(4°C overnight), GFP primary Antibody was diluted at 1:1000(4°C overnight),